Production of Recombinant Peanut Allergen Ara h 2 using Lactococcus lactis
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چکیده
منابع مشابه
Production of Recombinant Peanut Allergen Ara h 2 using Lactococcus lactis
BACKGROUND Natural allergen sources can supply large quantities of authentic allergen mixtures for use as immunotherapeutics. However, such extracts are complex, difficult to define, vary from batch to batch, which may lead to unpredictable efficacy and/or unacceptable levels of side effects. The use of recombinant expression systems for allergen production can alleviate some of these issues. S...
متن کاملRecombinant peanut allergen Ara h I expression and IgE binding in patients with peanut hypersensitivity.
Peanut allergy is a significant health problem because of the frequency, the potential severity, and the chronicity of the allergic sensitivity. Serum IgE from patients with documented peanut hypersensitivity reactions and a peanut cDNA expression library were used to identify clones that encode peanut allergens. One of the major peanut allergens, Ara h I, was selected from these clones using A...
متن کاملPeanut allergen (Ara h 1) detection in foods containing chocolate.
Inadvertent exposure to peanut in foods poses health risks for peanut-allergic individuals that can be reduced by improving detection systems for allergen contaminants in food products and manufacturing processes. Detection of peanut in chocolate has been especially difficult. We report the optimization of conditions for measuring a major peanut allergen, Ara h 1, in chocolate with the use of a...
متن کاملIgE cross-reactivity between the major peanut allergen Ara h 2 and the non-homologous allergens Ara h 1 and Ara h 3
BACKGROUND Ara h 1, a vicilin; Ara h 2, a 2S albumin; and Ara h 3, a legumin, are major peanut allergens. Ara h 2 is an important predictor of clinical reactivity to peanut, but cosensitization to all 3 allergens is correlated with the severity of patients' symptoms. OBJECTIVE We investigated whether cosensitization to these 3 allergens is caused by IgE cross-reactivity, despite the fact that...
متن کاملProduction of functionalized biopolyester granules by recombinant Lactococcus lactis.
Many bacteria are naturally capable of accumulating biopolyesters composed of 3-hydroxy fatty acids as intracellular inclusions, which serve as storage granules. Recently, these inclusions have been considered as nano-/microbeads with surface-attached proteins, which can be engineered to display various protein-based functions that are suitable for biotechnological and biomedical applications. ...
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ژورنال
عنوان ژورنال: Microbial Cell Factories
سال: 2007
ISSN: 1475-2859
DOI: 10.1186/1475-2859-6-28